Image by National Cancer Institute


On 15th Jan Stefanie gave her thesis presentation and defended sucessfully. Her PhD thesis title is

Co-infection of human cervix organoids by HPV and Chlamydia causes unique patterns of cellular reprogramming

Investigating Chlamydia, HPV and co-infection dynamics using human primary 3D cervical epithelial models

In this project, we aim to investigate the co-habitation and effects of HPV, Chlamydia and co-infections on the squamous and columnar epithelium of the cervix. Towards this we would first further optimize our method to produce high titers of native high-risk HPV using 3D organoid models for infection analysis. We will investigate how these complex 3D epithelial tissue models facilitate the life cycle of HPV and/or chlamydia, and how infections are established with respect to the type of cells they infect.

Wholemount staining of two-week-old ectocervical organoid immunolabelled for proliferating cells with Ki67 (red), actin cytoskeleton with Phalloidin (green), and nuclei (blue.