In this project, we aim to investigate the co-habitation and effects of HPV, Chlamydia and co-infections on the squamous and columnar epithelium of the cervix. Towards this we would first further optimize our method to produce high titers of native high-risk HPV using 3D organoid models for infection analysis. We will investigate how these complex 3D epithelial tissue models facilitate the life cycle of HPV and/or chlamydia, and how infections are established with respect to the type of cells they infect.